Lactase vs. Lactose

Overview

Objectives/Goals Determine if the amount of lactase has an effect on the process of breaking down sugar molecules in milk. Methods/Materials Procedural Method: 1.-3. Gather and label test tubes and put in racks. 4. In test tubes 1 and 2 pour 20 mL of whole milk. 5. Place 5g of lactase in test tube 2. 6. Pour 20 mL of distilled water into test tube 3 and put in 5g of lactase. 7. Pour 20 mL of corn syrup into test tube 4. 8.-9. Test tubes sit overnight and use chemstrips to detect sugar. 10. Fill a large beaker halfway with water, heat top. 11. Heat water to almost boiling on the burner. 12. Place all test tubes into the water bath. 13. Add 5 mL of Benedict#s solution to each test tube. 14. Record any color changes. 15. Repeat the project using 2g of lactase. Materials: 1. Balance; 2. 9 Beakers; 3. Benedict's solution; 4.Camera; 5. Goggles; 6. 9 graduated cylinders; 7. Grease pencil; 8. Burner; 9. Lactase; 10. 9 medicine droppers; 11. Whole milk; 12. 16 test tubes; 13. 4 test tube racks; 14. A pair of mittens; 15. Water; 16. Distilled water; 17. White corn syrup. Results The Amount of Lactose Detected-5g of Lactase Chem Strips Test Tube Content(s) Lactose mg/mL 1 20mL Whole Milk 100 2 20 mL Whole Milk-5g of Lactase 250 3 20mL Distilled Water-5g of Lactase Negative 4 20mL Corn Syrup 250 Benedict's Solution Test Tube Content(s) Color 1 20mL Whole Milk Orange 2 20 mL Whole Milk-5g of Lactase Yellow 3 20mL Distilled Water-5g of Lactas Negative 4 20mL Corn Syrup Brown

Competition history

• CSEF 2009 Biochemistry/ Molecular Biology · Entry J0407

Resources

• CSEF project summary ↗

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