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Isolation and Sequencing of Mitochondrial DNA

CSEF · 2002 Zoology

Mark A. Herron · La Sierra High School, Riverside

Overview

Objectives/Goals The objective in this project is to determine whether the amount of variation present within a population is sufficient to warrant a separation of the group into various species. Methods/Materials DNA was isolated, purified, quantified, and visualized. Once the DNA purity was varified, isolates were exposed to PCR condtions and specified primers were used to obtain an 1100bp sequence of the mitochondrial gerone. The sequences were then run throguh an ABI flourescent sequence. Lastly, the sequences were compared using various computer programs to analyze and determine the degree of variation. Results Ample variation was found to support the separation of the species into two distinct groups. Conclusions/Discussion Additional research must be done in order to support the findings from this preliminary project.

Summary statement

My project was about finding the genetic variation within a large group of iguanas

Competition history

  • CSEF 2002 Zoology · Entry S1910

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