Is There a Relationship Between the Disease Severity and the Actual Fungus Titer of the Innoculated Lettuce Plants?

Overview

Objectives/Goals I#m trying to figure out if there is a relationship between the disease severity of the fungus, Verticillium dahliae, on the inoculated lettuce plants compared to the actual fungus titer of the plants. My hypothesis is that there is a relationship between the disease severity and the actual fungus titer of the inoculated lettuce plants. I think that the higher the level on the disease severity scale the more fungus that plant actually has. Methods/Materials The materials I will be using are as follows:40 inoculated lettuce plants(12 post inoculated),5 moculated lettuce plants, petridishes, micro pipets , diH2O,scale(fresh weight),PDA (potatodextroseagar),refrigerator, plastic tubes, mortar, pestle, gloves, spatula/spreader, microscope, water & bucket ,and a colony counter, Using these materials I first washed the lettuce plant#s roots , cut off the foliage, then labeled them. I then weighed the roots. I cut off all of the linear roots , then I ground up each tap root with 1 ml of diH2O. After each root was ground I added 4 more ml of diH2O. Then using a micro pipet I took 1 ml of each tap root mixture and placed it into plastic tubes. I then made dilutions of each mixture. I spread 250 micro liters aliquots of each dilution onto PDA plates. I waited 4 days to count the colonies of Verticillium dahliae. I will then compare the colonies of Verticillium dahliae to the disease severity score I originally made. Results I found that there was a relationship.The relationship was there but I couldn't tell if there was a great one or barely there Conclusions/Discussion I fould that there was a relationship between the two. If I tested more inoculated lettuce plants the relationship would be more clear/

Competition history

• CSEF 2005 Microbiology · Entry S1316

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