Investigating the Effects of Carbon Dioxide and Temperature on Nannochloropsis oceanica Using a Hemocytometer Counting Method

Overview

Climate changes that include ocean acidification and global warming are serious problems in the ecosystem, affecting marine phytoplankton, including Nannochloropsis oceanica. In the effort to further explore the impact of rising temperature and carbon dioxide (CO2) concentrations on oceanic ecosystems, the phytoplankton Nannochloropsis oceanica was used as a model organism. This study explored the effect of temperature change and CO2 concentration on the growth of Nannochloropsis oceanica, achieving 243 samples that were tested with three different temperatures (24 degrees Celsius (°C), 28°C, 32°C) and CO2 flow rates (0 milliliter (ml)/min, 0.4 ml/min, 0.6 ml/min), utilizing a hemocytometer counting method. Results indicate that the CO2 concentration has a significant effect on the population of Nannochloropsis oceanica. But compared to the CO2, temperature didn’t affect a lot. The Nannochloropsis oceanica in the lowest temperature and highest concentration of CO2 in its environment had the highest population growth, and in the highest temperature and lowest concentration of CO2, it had the lowest population growth. Results show the serious negative effect of climate change on the ecosystem and the importance of environmental protection. Population blooms due to excess CO2 taking up ocean resources causing dangerous ecological imbalances.

Competition history

• ISEF 2025 Earth and Environmental Sciences · Entry EAEV009T

Resources

• ISEF abstract page ↗

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