In What Ratio Are Genes Passed On?

CSEF · 2018 Biochemistry/ Molecular Biology Second Award

Overview

Objectives/Goals The objective of this study is to determine the ratio of how genes are passed on and how often they physically appear in the offspring. Methods/Materials Wild type male C. elegans (roundworm), unc-3 mutated hermaphrodite C. elegans, rol-6 mutated hermaphrodite C. elegans, petri dish with NGM culture media, dissecting microscope, picker, incubator. Bred male wild type worms with a mutated hermaphrodite worm, counted phenotypes of offspring, allowed hermaphrodite offspring to self-fertilize, and counted phenotypes of the second generation of offspring. Conducted twice, once with unc-3 mutation and once with rol-6 mutation. Results The first generation of the unc-3 mutated hermaphrodites' offspring all showed wild type phenotypes, and the second generation showed a 1:3 phenotypic ratio of unc-3 to wild type. The first generation of the rol-6 mutated hermaphrodites' offspring all showed rol-6 phenotypes, and the second generation showed a 3:1 phenotypic ratio of rol-6 to wild type. Conclusions/Discussion The unc-3 mutation is recessive, while the rol-6 mutation is dominant, and it is possible to find the characteristics (dominance or recessiveness) of a gene through the phenotypes of heterozygotes with those genes.

Summary statement

I conducted an experiment to find a pattern of how genes are passed.

Help received

I used lab equipment in Rothman Lab in University of California, Santa Barbara under the supervision of Dr. Jeong.

Awards (1)

Competition history

  • CSEF 2018 Biochemistry/ Molecular Biology · Entry J0509

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