In vitro Evaluation of the Antagonistic Potentials of Two Trichoderma Species Against Different Fusarium Isolates to Assess Isolate Dependent Responses in Plant Disease Suppression

Overview

Fusarium species are plant pathogens that reduce crop yields and contaminate food with harmful mycotoxins. Because long-term reliance on chemical fungicides can lead to resistance and environmental concerns, alternative control strategies are needed. In this study, I evaluated the antagonistic potential of two Trichoderma species against 50 Fusarium isolates under controlled in vitro conditions. A dual culture assay was conducted on Potato Dextrose Agar, and Fusarium radial growth was measured on days 3, 6, and 9 to determine inhibition relative to control plates. By Day 9 most isolates showed between 40–65% growth inhibition, with maximum suppression reaching approximately 78% in highly responsive isolates. However, responses were not uniform. A small number of isolates exhibited lower inhibition (approximately 22–30%) or partial recovery over time, indicating that antagonistic interactions are isolate-dependent and dynamic. Statistical analysis using a one-tailed paired t-test confirmed significant growth reduction, and large Cohen’s d values reflected strong biological effects. These results demonstrate consistent but variable suppression of Fusarium and highlight the importance of screening multiple isolates when evaluating biological control agents.

Awards (2)

• King Abdulaziz & his Companions Foundation for Giftedness and Creativity: Full Scholarship from King Fahd University of Petroleum and Minerals(KFUPM) (and a $400 cash prize) $400
• King Abdulaziz & his Companions Foundation for Giftedness and Creativity: NOT TO BE READ -- $400 cash prize for each Full Scholarship from King Fahd University award recipient $400

Competition history

• ISEF 2026 Plant Sciences · Entry PLNT009

Resources

• ISEF abstract page ↗

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