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Identification and Characterization of Antibiotic-Resistant Bacteria

JSHS · 2025

Sahira Bhakta, Zulma Perez-Estrella · Shawnee Mission Northwest High School, Shawnee, KS

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Overview

This research project aims to investigate the presence and diversity of Tetracycline -resistant bacteria in soil samples collected from local playgrounds, focusing on understanding the potential risks associated with antibiotic resistance in urban environme nts. Tetracycline, a widely used antibiotic, has been implicated in the spread of resistance among environmental bacteria, and urban playgrounds may serve as reservoirs for resistant strains due to human activity and contamination from various sources. Soi l samples will be collected from multiple playgrounds across the Johnson County, Kansas area, and the presence of tetracycline-resistant bacteria will be assessed using culture -based techniques, followed by DNA plasmid extraction and concentration readings , gram -staining, and genome sequencing. Results of high antibiotic resistance activity within each playground's samples. Specific bacterial types will continue to be analyzed through BLAST Genome Sequencing. Findings will likely underscore the potential health risks associated with the spread of resistance in public spaces and emphasize the need for monitoring and controlling antibiotic contamination in urban environments to mitigate the emergence of resistant bacterial and fungal strains.

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Competition history

  • JSHS 2025 Category not listed

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Source: Junior Science and Humanities Symposium

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