How does APC influence CAF and Tumor Cell Interaction

Overview

Triple Negative Breast Cancer (TNBC), named after all three negative receptors, is a dangerous subtype of breast cancer (Grasset, Eloïse). My experiment focuses on TNBC cells that have the tumor suppressor, adenomatous polyposis coli (APC). It also focuses on cancer associated fibroblasts (CAFs) due to their abilities to increase tumor cell interactions. In my studies, I will use them to make a CAF-conditioned media to place over the cells. Ultimately, I will determine if the tumor cell interactions (prolif eration and migration) will be influenced when CAF -conditioned media is placed onto the APC –KO cell lines. If you add CAFs to APC -KO cell lines then you will have an increase in tumor cell interaction because when CAFs are introduced to cancer cells, the tumor cell interactions will increase. To answer my question, I performed a proliferation assay, migration assay, and western blot. The proliferation determined how quickly the cells reproduce, the migration determined how quickly the cells reconnected when split apart, and the western blot determined expression for both Cyclin D1 and STAT3 proteins. My graphs overall had no significance. This means no samples had a noticeable change. Specifically, there was no change in the tumor cell interaction of the APC-KO cell lines when CAF -conditioned media was added which proved my hypothesis incorrect. With this knowledge, I would like to expand on my research by discovering why there was no significance and treat my cells to a chemotherapy drug before the experiment to determine if there is a significant change after.

Competition history

• JSHS 2025 Category not listed

Resources

• National JSHS abstract book ↗

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