Growth of Water Bottle Bacteria

Overview

Objectives/Goals The goal of my project is to find out which environment (in a car, on a kitchen counter, on an outside surface, or in the refrigerator) makes a Crystal Geyser Natural alpine spring water bottle grow the most bacteria after being drunk out of. Methods/Materials In my project, the main materials I used were: Crystal Geyser water bottles, sterile cotton swabs, petri dishes, agar, magnifying glass, a lamp, and a human mouth. To do my project I drank from each water bottle, then took a sample from the inside rim of the bottle. Then I had the bottles sit in their environment. After 2 days, and again after 5 days, I sampled the rim and the water itself. Then I measured the amount of bacteria that grew in each petri dish. Results Of all the samples I took, after five days, the results were that the outside environment had the most bacteria. The car environment had the next most, then the kitchen counter, and last the refrigerator. Conclusions/Discussion In conclusion, my hypothesis was partially correct. I was wrong when I predicted the most bacteria would grow in the bottle in the car. Instead the most bacteria grew in the outside environment. However, I was correct about which environment would grow the least bacteria..... the refrigerator.

Competition history

• CSEF 2003 Microbiology · Entry J1307

Resources

• CSEF project summary ↗

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