Growing Algae in Different Conditions

CSEF · 2019 Earth & Environmental Sciences

Overview

Objectives The objective of this project is to test whether or not a supply of different concentrations of carbon dioxide, fertilizer and increased light exposure will increase algae growth, which can be useful in many ways such as producing oxygen for breathing. Based on the conducted research, it is hypothesized that the fertilizer will have the biggest effect on the algae growth. To conduct the experiment, 50 ml of cultured algae was tested for growth under different conditions for 1 month: three different concentrations of CO2, (5%, 10%, 20%), three different concentrations of fertilizer (5%, 10%, 20%), and continuous exposure to light. Sugar and brewer s yeast were used to produce CO2 in reactor bottles to be released to the bottles labeled CO2, through a tubing apparatus connecting both bottles. A total of 40 bottles of algae were cultured (5 for the control, 5 for each of the CO2 concentrations, 5 for each of the fertilizer concentrations, and 5 for continuous exposure to light). Algae growth was analyzed using spectrophotometer. The results showed that the 20% carbon dioxide concentration was the best for the algae to grow. Additionally, the algae grew with the higher concentration of fertilizer at a slower rate. Exposure to continuous direct light significantly decreased the amount of algae. Therefore, my hypothesis was proven incorrect in that fertilizer did not have the best effect on algae growth. The least effective was the control as it had no source of CO2 or fertilizer. To further expand this project, I would like to test if different temperatures, level of pH of the water, and/or if vitamins, such as VIT B, VIT C, and VIT E are provided, would affect algae growth. Methods 50 ml of cultured algae was tested for growth under different conditions for 1 month: three different concentrations of CO2, (5%, 10%, 20%), three different concentrations of fertilizer (5%, 10%, 20%), and continuous exposure to light. Sugar and brewer s yeast were used to produce CO2 in reactor bottles to be released to the bottles labeled CO2, through a tubing apparatus connecting both bottles. A total of 40 bottles of algae were cultured (5 for the control, 5 for each of the CO2 concentrations, 5 for each of the fertilizer concentrations, and 5 for continuous exposure to light). Algae growth was analyzed using spectrophotometer. Results

Summary statement

The objective of this project is to test whether or not a supply of different concentrations of carbon dioxide, fertilizer and increased light exposure will increase algae growth.

Help received

I have conducted the experiment myself.

Competition history

  • CSEF 2019 Earth & Environmental Sciences · Entry J0902

Resources

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