Ginger Root Bioactive Compounds Specifically Inhibits Growth of Colon Cancer Cells in Culture

Overview

Colon cancer is affluent and greatly impacts the lives of many. Ginger is a common food, yet its health benefits as a whole food and 6-gingerol, as one of its bioactive compounds in prevention of colon cancer, have not been fully addressed. This experiment investigated the effects of ginger j uice and 6-gingerol on colon cancer cell growth and death. Fresh ginger roots were homogenized for juice preparation. Colon cancer SW480 cells and normal colon epithelial cells CCD -18Co were treated with ginger juice and/or 6 - gingerol. Cell metabolic activity, cell apoptosis, and cell cycle arrest were accessed. Data were analyzed by two-way ANOVA with a Tukey post-hoc test and statistical significance was set at p < 0.05. The results showed that ginger juice selectively inhibited SW480 cell growth at 25 µL/mL for 40 hrs. 6 -gingerol specifically inhibited SW480 cell growth at 0.5 µmoL/L (p < 0.01). More than 1 µmoL/L 6 -gingerol did not give more power to inhibit SW480 cell growth. CCD -18Co cell growth rates were not changed after 6 - gingerol treatments. Imm unoblotting results revealed that the elevation of Myt1 levels and decreases in CDK1, p21 Wafl/Cip1 and pSer642 -Wee1 only occurred in SW480 but not CCD -18Co cells. Taken together, 6-gingerol can specifically inhibit SW480 cancer cells without killing normal CCd -18Co cells through cell cycle arrest. Ginger juice can selectively inhibit colon cancer cell growth.

Competition history

• JSHS 2024 Category not listed

Resources

• National JSHS abstract book ↗

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