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Extracellular ATP Effects on Mac-T Cell Actin Distribution

JSHS · 2025

James Peng, David McClenahan · Cedar Falls High School, Cedar Falls, IA

Overview

Leukocyte migration into the bovine mammary gland is a hallmark sign of mastitis. The McClenahan lab has previously shown that the presence of extracellular ATP is associated with permeability changes in epithelial monolayers (Mac -Tcells). It is thought t hat permeability changes are associated with actin cytoskeletal changes within the cell. To test this hypothesis, I treated Mac-Tcells with various concentrations of ATP, after which a commercial kit was used to extract the globular/filamentous forms of a ctin. Protein electrophoresis followed by western blot comparison of band density using the software Image -Jwas then used to determine actin levels under the different treatment conditions. Significant changes in band densities were determined using ANOVA followed with a post - hoc analysis. Mac-Tcell exposure to concentrations of ATP from 0.1 millimolar to 20 millimolars resulted in significant increase in G -actin levels within the cell. Overall, it appeared that exposure to extracellular ATP may be a mechanism that resulted in cytoskeletal changes in barrier epithelial cells, potentially assisting leukocyte movement into the lumen of the gland.

Competition history

  • JSHS 2025 Category not listed

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Source: Junior Science and Humanities Symposium

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