fairfolio
Saved Projects

Expression and Characterization of Tardigrade CAHS Protein Q

AJAS · 2026

Brenda Randell · North Carolina Academy of Science

Overview

The tardigrade protein CAHS D has been shown to stabilize proteins such as lactate dehydrogenase at high temperatures. This experiment focused on the stabilization abilities of another CAHS isoform, CAHS Q, on alcohol dehydrogenase, another oxidoreductase enzyme. Alcohol dehydrogenase is used to convert ethanol into acetaldehyde, and vice versa. CAHS Q’s expression was optimized to be 250 µM final IPTG incubated at 16°C for 16 hours. This was confirmed by SDS-PAGE analysis. This condition was then used for the protein’s FPLC purification in which the positive CAHS Q protein was subjected to anion exchange chromatography. The procedure purified the protein, which was then dialyzed and lyophilized.

Competition history

  • AJAS 2026 Category not listed

Related projects

Closest projects by meaning, across every fair and year in the corpus.

Browse more like this

Source: AAAS Annual Meeting (Confex) / American Junior Academy of Science

Save projects to your library

Sign in with Google to keep track of projects you find interesting, organized into folders. An account also raises your daily allowance for “Has this been done?”, and lets you create a key for the MCP server with a much higher limit than anonymous use. Browsing stays public.

Continue with Google