Engineering Pink Salt
CSEF · 2013 Microbiology (General) First Award
Overview
Objectives/Goals Create an open Halobacteria plasmid with do-it-yourself (DIY) methods Methods/Materials Materials--- Strains- E. coli K12 ER2267, Halobacteria NRC-1 Plasmids- pGreen, pBeloBac11, pUC19, pUC57 + insert, Chemicals / media DMSO, LB agar, Agarose, ethidium bromide, Bromophenol Blue/Xylene Cyanol Gel Loading Buffer, Distilled water, Epsom Salt, PEG 3350 (miralax), LB broth, Halobacteria broth, Halobacteria agar, Ampicillin, chloramphenicol, CaCl, Gycerol, Tools Electrophoresis box, Transilluminator, Power supply, loops, Bunsen burner, pipettes, water bath, centrifuge, PCR machine, Vortex, refrigerator, freezer, glasses, Expendables Inoculating loops (plastic), petri dishes, PCR tubes, Centrifuge tubes, Culture tubes, gloves, masks, Results All polymerase reactions were verified by electrophoresis. The projects DNA could not be because of minimal amounts (gibson assembly). No E coli colonies observed. Halobacteria colonies were observed. Conclusions/Discussion All of the Polymerase chain reactions worked. The actual DNA could not be verified because of minimal amount of it. However, a streak colony was observed on one of the transformed plates. Since it was small, and salt
Summary statement
Creating a shuttle vector for genetically modifying the thrid domain of life, Archaea.
Help received
Went to LA biohackers for help with ethidium bromide, verification of PCR. Used my own electrophoresis equipment, used their transilluminator. Needed to go there for my fair's regulations. Everything else I did
Awards (1)
Competition history
- CSEF 2013
Resources
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Source: California Science & Engineering Fair public projects