Effects of Estrogen and Phytoestrogen on MCF-7 Human Breast Cancer Epithelial Cells

Overview

Phytoestrogen is a polyphenolic compound naturally present in plants. Recent research presents that due to the structural similarity of phytoestrogen to estrogen, when administered into the animal systems, phytoestrogen mimics circulating estrogen in the mammalian reproductive system. However, the effects of this plant hormone in animal cells when co-present with estrogen have not been studied. In this project, we investigated the effects of estrogen, phytoestrogen, and estrogen – phytoestrogen mixtures on the viability of human MCF-7 breast epithelial cells. The effect of different concentrations of estrogen alone (0.1, 1, and 10 μM), phytoestrogen alone (0.1, 1, and 10 μM) and estrogen and phytoestrogen combinations (E10+P10, E10+P1, E10+P0.1, E1+P1, E1+P10, E1+P0.1, E0.1+P10, E0.1+ P1, E0.1+P0.1 μM) on cell viability were tested by using MTT cell viability test at three different time points. The results showed that, at lower concentrations, phytoestrogen increased cell proliferation. The effect of phytoestrogen on cell viability seemed to be opposite of the effect of estrogen alone. The effect at seventy-two hours followed the normal trend of estrogen only at the E1(10 μM) and phytoestrogen combination. The rest of the combination doses for other estrogen concentrations were opposite of estrogen alone. The overall effect of phytoestrogen and estrogen combinations should be further investigated.

Competition history

• AJAS 2019 Category not listed

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