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Effect of Storage Time and Temperature on Norovirus Positive Stool Specimens Collected on Whatman Flinders Technology Associates Elute Cards

JSHS · 2020

Logan Ridenbaugh · Ramstein High School Ramstein, Germany

Overview

Dr. Cole Anderson & Mr. Fritz Castillo Landstuhl Regional Medical Center Michelle Harrington Ramstein High School Traveler's diarrhea (TD) remains a significant burden to operational readiness during military deployment and overseas travel. Pathogen detection is limited to Polymerase Chain Reaction (PCR) detection in advanced laboratories and requires immediate processing or cold-chain management of stool samples. In this study, the researcher investigated the performance of Whatman Flinders Technology Associates Elute cards (FTA cards) in stabilizing Norovirus (NoV) positive stool samples for PCR detection at room temperature and 37° Cfor 1 week, 1 month, and 2 months. Detection rates and cycle thresholds (Ct) were compared between frozen and FTA card stored samples using NoV-specific PCR. Overall, the researcher did not detect a significant difference in positivity rates between frozen and FTA card stored samples. When comparing Ct values, prolonged 37° Cexposure for 2 months caused Ct values to increase, notably a significant difference was observed between room temperature (RT) 1-month and 37° C 2-month storage conditions. The findings support the use of FTA cards for storage of stool samples and advancing the usage for molecular based pathogen detection.

Competition history

  • JSHS 2020 Category not listed

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Source: Junior Science and Humanities Symposium

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