Eco-Friendly Membrane Technology: Unlocking Antibacterial Potential of Biowaste Materials Against Foodborne Pathogens

Overview

Numerous studies have investigated the use of natural compounds for antibacterial applications. This study evaluated biowaste materials such as morro seed, moringa seed, strawberry seed, and citrus peel albedo for their antibacterial potential. The materia ls were subjected to hot water or acidic treatments, followed by extraction using membrane technology, and then tested against four major foodborne pathogens: Escherichia coli O157, Listeria monocytogenes , Salmonella typhimurium, and Staphylococcus aureus. Citrus peel albedo extract demonstrated strong antibacterial activity, effective at concentrations as low as 0.032 mg/ml across all bacterial strains. Moringa seed extract showed selective activity against Gram -positive bacteria with minimum inhibitory concentration (MIC) at 2,500 mg/ml and minimum bactericidal concentration (MBC) at 5,000 mg/ml. However, extracts from morro and strawberry seeds showed no antibacterial activity even at concentrations as high as 200,000 mg/ml. This research underscores the promise of biowaste materials as viable antibacterial agents, contributing to food safety and public health, while also promoting environmental sustainability through eco -friendly membrane technology in place of conventional solvent extraction methods. Mitochondrial Ribosomal Protein -47 (MRPL47) identified as a diagnostic biomarker of ovarian cancer Meenakshi Pradeep New Berlin Eisenhower, New Berlin, WI MRPL47 (Mitochondrial Ribosomal Protein Large Subunit 47) gene encodes a protein that is part of the large subunit of the mitochondrial ribosome. MRPL47 is located in the 3q26 locus of chromosome-3, a chromosomal position frequently amplified in ovarian cancer. MRPL47 is often highly amplified, or exhibits copy number gain as part 3q26 amplicon in ovarian cancer, which in turn resulted into an increase in the expression of MRPL47 mRNA and MRPL47 protein in ovarian cancer patients. Importantly, high expression of MRPL47 is correlates with poor level of survival of ovarian cancer patients. Gene set enrichment analysis and target specific knockdown assays reveal that MYC transcription factor regulates MRPL47 expression. Our analysis further identified MRPL47 is blood samples, with significantly higher expression levels in plasma collected from ovarian cancer patients compared to those of healthy volunteers . Taken together, we demonstrate that MRPL47 can be used as a diagnostic biomarker for ovarian cancer and other cancers encompass 3q26 chromosomal amplification.