Doorknobs of Disease

Overview

Objectives/Goals Do medical office doors carry staph? I hypothesized that at least 3 out of 10 samples would test positive for staph. Methods/Materials Nutient agar, petri dishes, sterile swabs, distilled water, log book. 20 samples were collected by swabbing local medical office doorknobs. Samples were swabbed into petri dishes and placed in a room temperature location. Daily observations were written in the log book. I had planned to have a microbiology lab test samples but when I called to confirm I was told that I needed to have a current TB test. I was not able to get a TB test in time befoe the sciend fair due date. Results Visually 4 out of 10 samples had growth on my first group of samples. In the second group of samples 9 out of 10 had growth.Bonnie from the lab suggested I do visual obeservations and a gram stain.I also did a gram stain and 1 sample tested gram negative which means it's not staph but it could be strep. Conclusions/Discussion Unfortunately I was not able to go into the microbiology lab due to regulations. I was able to conduct a 1 gram stain and found it to be gram negative.

Competition history

• CSEF 2010 Microbiology · Entry J1731

Resources

• CSEF project summary ↗

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