fairfolio
Saved Projects

Colorectal Cancer: Vitamin D Receptor Deficiency Upregulates Expression of Claudin 5

ISEF · 2014 Mathematics

Zhang, Weipeng

Is this your project?

Overview

In this project, the relationship between vitamin D receptor (VDR) and Claudin 5 with regards to colorectal cancer is investigated. I will compare the expression levels of the Claudin 5 protein and gene in wildtype mice and mice with the vitamin D receptor gene knocked out (no expression of vitamin D receptors) to support the hypothesis that vitamin D receptor deficiency induces increased expression of the Claudin 5 protein. Procedures: Immunofluorescence: I Deparaffinized colon sections, stained them with antibodies, and observed and compared expression levels of Claudin 5 in different sample groups. Western Blot: I culture cells, lysed them, and loaded samples into gel and run gel electrophoresis, transferred protein onto nitrocellulose membrane, incubated in antibody, and detected the protein. Real-time polymerase chain reaction (qPCR): I extracted RNA, ran reverse transcription PCR, and ran qPCR. Results showed that Claudin 5 exists in higher amounts inside mice colon belonging to VDR knockout mice compared to wildtype based off of immunofluorescence results. Results also showed that the Claudin 5 protein and gene is found to be expressed in significantly higher amounts in mouse embryonic fibroblast cells when the VDR gene is knocked out compared to wildtype mouse embryonic fibroblast cells (p = 0.03 for Western Blot, p=0.008 for qPCR). Claudin 5, which promotes the metastasis of colorectal cancer cells, is upregulated by low expression levels of VDR. It is also possible for Claudin 5 to be upregulated by low levels of VDR in colorectal cancer patients. Further research in the future concerning VDR and Claudin 5’s mechanism in colorectal cancer may be done in order to eventually find a treatment and alleviate the pain of so many people in the world today.

Working on something like this? See what's been done and what's still open.

Competition history

Resources

Related projects

Closest projects by meaning, across every fair and year in the corpus.

Browse more like this

Report a problem

Source: Regeneron International Science and Engineering Fair

Save projects to your library

Sign in with Google to keep track of projects you find interesting, organized into folders. An account also raises your daily allowance for “Has this been done?”, and lets you create a key for the MCP server with a much higher limit than anonymous use. Browsing stays public.

Continue with Google