Catobolic Pathways in Saccharomyces cerevisiae

CSEF · 2008 Biochemistry/ Molecular Biology

Overview

Objectives/Goals Observing the amount of carbon dioxide released through fermentation in Saccharomyces cerevisiae, or baker's yeast, when white sugar, honey, and brown sugar are given. It was hypothesized that becuase honey is composed of mainly simple sugars the Saccharomyces cerevisiae will be able to consume honey more easily and thus create a greater amount of carbon dioxide. Methods/Materials Saccharomyces cerevisiae placed into test tubes and activated with water, either white sugar, brown sugar, or honey is given to the yeast. The test tube is then sealed with a one-way valve and placed into a water displacement apparatus. Carbon dioxide is caught in beakers and measured. Results After multiple trails, it was found that honey produced the least amount of carbon dioxide. The yeast given white sugar produced the most amount of carbon dioxide, and the yeast with brown sugar produced the second most amount of carbon dioxide. Conclusions/Discussion The results was ironic becuase I predicted that honey would produce the most amount of carbon dioxide and white sugar would create the least amount of carbon dioxide. Based on the results, it can be seen that refined sugars are better for yeast to produce carbon dioxide.

Summary statement

The efficiency of different forms of glucose in the fermentation of yeast.

Help received

The Carter High School science department for lending me the equipment needed to make the water displacement apparatus.

Competition history

  • CSEF 2008 Biochemistry/ Molecular Biology · Entry S0406

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