Calpains 8/9 Areexpressed in Murine Intestines;Regulated by wnt3a in Coloncancercells

Overview

Calpain 8 and calpain 9 are calcium dependent proteases, predominantly expressed in the stomach. They are reported to be implicated in processes such as cancer development and progression. Upregulation of calpains have been seen in tumors and cancers including gastric cancer, breast, and ovarian cancer suggesting its activity in cancer development. However, their activity in colon cancer remain unknown. To investigate a potential role in colon cancer, we have stimulated human colon cancer cell line with WNT3A, Tumor Necrosis Factor alpha (TNF) and both for 24 hours. After WNT stimulation, the relative mRNA expression of calpain-8 and calpain-9 significantly increased, whereas TNF did not affect the expression of calpain-8 and 9. However, when stimulated with both; TNF has suppressed the ability of WNT to upregulate, suggesting an interaction between the two. We also found that calpain-9 to be highly expressed in murine colon through our immunohistochemical analysis. Taken together, our result suggest a possible role for calpains-8 and 9 that warrants further investigation.

Competition history

• AJAS 2020 Category not listed

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