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BRCA1 Methylation in Sporadic Breast Cancer

CSEF · 2007 Biochemistry/ Molecular Biology

Nelli Ghazaryan · Bravo (Francisco) Medical Magnet High School, Los Angeles, Los Angeles County

Overview

Objectives/Goals To determine the stage in cancer in which there is a loss of BRCA1 expression, what the loss of this expression is due to. And what the effects of this loss are on the developement of sporadic breast cancer. Methods/Materials Throough Immuno Histo Chemistry, Hemotoxin & Eosin staining, Laser Capture Microdissection we will examine our collected multicutural cohort of patients, anaysizing tumor, lymph node and normal breast tissue for each. Using a nested PCR approach we will distinguish whether the patients have a loss of the BRCA1 protein or not. Results Distinctions between the patients who had a higher tumor grade percentage and the BRCA1 loss of expression were seen, as well as the effects of the BRCA1 methylation on the growth of tumors. Conclusions/Discussion INCONCLUSIVE DATE At this point many of the procedures and protocols are being optimized for study and patient samples are being garnered.

Summary statement

This project is about looking at BRCA1 loss abd nethylation and their correlation with the developement of sporadic breast cancer.

Help received

Research was done at USC Norris in the labaratory of Dr. Michael Press, under the mentorship of Dr. Melinda Epstein

Competition history

  • CSEF 2007 Biochemistry/ Molecular Biology · Entry S0405

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