Bean Plants and the Amount of Chlorophyll They Have with Different Types of Soils

Overview

Objectives/Goals My project was to determine if different types of soils have an effect on the amount of chlorophyll in pinto bean plant. Methods/Materials First, I planted nine plants,three tests in each type of soil. After fifty-nine days, I took a nine centimeter square piece leaf and crushed it. I added 20 milliliters of rubbing alcohol and boiled each solution in a test tube to obtain a chlorophyll solution. I filtered each solution to get rid of any leaf fragments and poured it into a small bottle to allow the solution to cool. I placed each sample in the spectrophotometer, which gave me a transmittance reading equal to the amount of chlorophyll in the solution. I measured the amount of chlorophyll at both a 660-nanometer and 440-nanometer wavelengths. I graphed and charted all of my results and compared to make my final conclusion. My materials were: nine pinto bean seeds, spectrophotometer, seventeen test tubes, three ring stands, heat plate, glass pot, 4 test tube clamps, rubbing alcohol, Whitney Farms Potting Soil, Whitney Farms Seed Starting Mix, and my Backyard Soil. Results The bean plants planted in Whitney Farms Potting Soil had the most chlorophyll, the beans plants planted in My Backyard Soil (mostly moist sand) had the second greatest amount of chlorophyll, and the bean plants planted in Whitney Farms Seed-Starting Mix had the least amount of chlorophyll. On average the bean plants planted in Premium Potting Soil had 36.7 at a 440 wavelength and at a 660 wavelength it had 98.03. On average, the amount of chlorophyll that the bean plants planted in Seed-Starting Mix had is 29.5 at a 440 wavelength and had 49.13 at a 660 wavelength. On average, the amount of chlorophyll and the bean plants planted in Backyard soil (mostly moist sand) had are 33.7 at a 440 wavelength and had 98.03 at a 660 wavelength. Conclusions/Discussion My conclusion is that the bean plants planted in Whitney Farms Potting Soil had the most chlorophyll, the beans plants planted in My Backyard Soil (mostly moist sand) had the second greatest amount of chlorophyll, and the bean plants planted in Whitney Farms Seed-Starting Mix had the least amount of chlorophyll. So, my hypothesis was incorrect; although these results were not significantly different.

Competition history

• CSEF 2005 Plant Biology · Entry J1638

Resources

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