Bacillus cereus + Essential Oils = ???

CSEF · 2006 Microbiology

Overview

Objectives/Goals The purpose of this project is to determine the effectiveness of different concentration levels of select essential oils on the growth of the bacteria, Bacillus Cereus. Methods/Materials 40 petri dishes were placed into groups of 5. lst group: one drop of first essential oil added/mixed into nutrient agar, 2 drops added/mixed to each of 2nd group, 3 drops added/mixed into 3rd group, 4th group: no essential oil added (controlled variable). Repeated using second essential oil. Inoculated all petri dishes with Bacillus Cereus spores and incubated 56 hrs at 33 degrees Celcius. Colonies were then counted. Results Thyme oil (2nd essential oil) resisted the growth of the bacteria with only one drop, whereas the Peppermint oil (lst essential oil) could only inhibit the growth of the bacteria at the highest concentration. Conclusions/Discussion My hypothesis was correct. I concluded that the Thyme oil had great effectiveness against the bacteria, but at the highest concentration, Peppermint oil inhibited the growth of Bacillus Cereus just as well.

Summary statement

My project is designed to test the effectiveness of different concentration levels of select essential oils on the growth of Bacillus Cereus.

Help received

Mother helped find reference books and supervised my experiment.; D. Cornelius helped find reference books; B. Peterson loaned lab equipment to me.

Competition history

  • CSEF 2006 Microbiology · Entry J1332

Resources

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Source: California Science & Engineering Fair public projects

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