Agro-Rhizoremediation Rhizoremediation with Agro-transformed Oryza sativa to Facilitate Arsenic Degradation in-situ
JSHS · 2023
Overview
The World Health Organization reports that arsenic contamination of soil and water is considered a global issue, threatening the health of over 150 million people worldwide. Arsenic accumulation in Oryza sativa (rice), in particular, is becoming increasingly problematic because of the repeated use of arsenic-laden groundwater for rice cultivation. The Environmental Protection Agency has set a limit of fewer than 20 parts per million (ppm) for arsenic in soil and less than 0.01 ppm in drinking water. However, current solutions to remediate arsenic are expensive and ineffective. Rhizoremediation is a promising technique to detoxify soil arsenic in situ, but it is not 100% effective, and arsenic still escapes into the edible parts of the plant. Therefore, in addition to remediating arsenic from the soil, it is vital to prevent arsenic accumulation in the rice grain. Therefore, this project aims to develop a joint procedure using rhizoremediation along with transgenic rice plants that have been genetically transformed with arsenic-resistant genes to facilitate self-detoxification. Raw sequences of 16s rRNA from 82 soil samples obtained from the National Center for Biotechnology Information were processed through Qiime2 to examine bacterial taxonomy in arsenic-contaminated and uncontaminated soil rhizospheres. Pseudomonas and Burkholderia genera constituted over 63% of the microbial community in the contaminated rhizospheres. Agrobacterium tumefaciens was transformed with acr3 and arsC arsenic resistance and reduction genes, respectively. Transformed A. tumefaciens was used to infect wounded rice cotyledons, which were then cultivated in plant tissue culture media to develop transgenic rice plants. Finding an Accessible, Environmentally Friendly Solution to Water Purification: Testing Aloidendron barberae and Bambusa dolichomerithalla as Natural Coagulants on Copper Ion Concentration Using UV-vis
Competition history
- JSHS 2023
Resources
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