The Effect of Dectin-1 on Trained Immunity and Overall Immune Response of Macrophages

Overview

Cancer is a devastating and major problem that is difficult to treat. A new and promising idea of trained immunity suggests that following stimulation with an antigen/pathogen, innate immune cells will have increased responsiveness to subsequent challenges . Macrophages are innate immune cells that are important to the innate immune system. WGP is a type of β-glucan, a fiber recognized as an inducer for trained immunity with 4 receptors. One of these receptors is Dectin- 1. In order to determine the role of Dectin-1 in trained immunity and overall immune response, bone marrow-derived macrophages were collected from mice and divided into four groups: WGP- trained Dectin-1 knock-out (WGP KO), untrained Dectin-1 knock-out (UNT KO), WGP-trained wild type mice (WGP WT), and untrained wild type mice (UNT WT). They were compared through the expression of CD80, CD86, MHC class II, and TNF -a, molecules that are released when macrophages are activated (activation markers). Though there was generally higher expression of t he activation markers following WGP training, expression (and therefore activation) was significantly greater for the wild-type macrophages. Even without WGP stimulation, the wild-type macrophages generally had higher activation marker expression. These fi ndings indicate that WGP-trained immunity largely depends on the Dectin -1 receptor, and Dectin -1 is critical for the optimal activation of macrophages. This study advances the understanding of how β -glucan- mediated trained immunity can be used for cancer immunotherapy. Louisiana

Competition history

• JSHS 2025 Category not listed

Resources

• National JSHS abstract book ↗

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