Optimizing hypoxia and reoxygenation protocol to study the molecular mechanism of oxygen- glucose deprivation resistance phenomenon in Arctic Ground Squirrel Neuron Progenitor Cells

Overview

Department This paper measures neurons’ viabilities after hypoxia and reoxygenation to optimize the protocol for Arctic Ground Squirrel Neuron Progenitor Cells (AGS NPCs), which can survive oxygen and glucose deprivation (OGD) - in-vitro model of ischemic stroke. Human neuron progenitor cells, however, are unable to sustain OGD like AGS NPCs. This OGD resistance phenomenon in AGS NPCs can be applied to treat ischemic stroke although its underlying mechanism is not well understood. To study the phenomenon, we need a working hypoxia-reoxygenation protocol (referred to as hypoxia protocol from this point in the paper), which means that there is a significant decrease in viability after treatment. Therefore, we have obtained N2A mouse neuroblastoma cells to test our hypoxi a protocol. N2A was expanded and differentiated following AGS NPCs expansion and differentiating protocol, then the experimental group was put in a hypoxia chamber for 24 hours, followed by reoxygenation for 24 hours. An Alamar Blue assay was done for both control and experimental group 3 times: before the hypoxia treatment, 24 hours after the hypoxia treatment, and 24 hours after reoxygenation. Our hypoxia treatment results in significant decrease in cell viability which implies significant cell death afte r reoxygenation period. Though an additional cell death assay must be done to quantify the cell deaths caused by our hypoxia protocol, the successful result coming out from this experiment means that we can use this protocol for rat neural stem cells and A GS NPCs for the study to investigate the role of neurogenesis in AGS NPCs OGD resistance.

Competition history

• JSHS 2024 Category not listed

Resources

• National JSHS abstract book ↗

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